Soybean Glycine max L. Nodules initiated with either type of strain were normal in appearance and demonstrated nitrogenase activity acetylene reduction. Regardless of the bacterial strain used for inoculation, the nodule cytosol and the cell-free extracts of the leaves contained both nitrate reductase and nitrite reductase activities. The nitrogenase activity of detached nodules initiated with the nitrate reductase-negative mutant strains was less affected by the KNO 3 treatment as compared to the wild-type strain; however, the results were less conclusive than those obtained with the isolated bacteroids. Addition of 0.
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The Murdoch University Research Repository is an open access digital collection of research created by Murdoch University staff, researchers and postgraduate students. Chen, Yung-Pin Pathways of aromatic metabolism in Rhizobium. PhD thesis, Murdoch University. Studies on the oxidation of a variety of aromatic substrates by Rhizobium trifolii TA1 and Rhizobium leguminosarum MNF indicated that the former could oxidise aromatic substrates normally leading to the catechol or protocatechuate branches of the 3-oxoadipate pathway, while the latter could not use substrates like benzoate requiring the catechol branch. Assays for typical enzymes of the catechol and protocatechuate branches confirmed this conclusion.
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